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Ponceau S solution has been used for membrane staining (Western blotting). The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate.

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PONCEAU S STAINING SOLUTION. Sigma Prod. No. P For Cellulose Acetate Protein Detection and Western Blot Total Protein Detection.

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Search results for PL at Sigma-Aldrich. Ponceau S solution. 1 Product Result Properties. Empirical Formula (Hill Notation): C22H12N4Na4O13S4.

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Ponceau S stain solution (Sigma #P). Nylon transfer membrane (Millipore). IPTG stock solution (1 M in water; Fisher #). • Chemiluminescence.

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Ponceau S solution. Product Number.: P Brand.: Sigma-Aldrich. REACH No.: A registration number is not available for this substance as the substance.

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); Ponceau S solution (Sigma, cat. no. P); BSA powder; Tween 20 ( Amresco, cat. no. M); enhanced chemiluminescence (ECL) solution (GE.

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Ponceau S solution (% (w/v) in 5% acetic acid) (Sigma-Aldrich, St. Louis, MO, USA, P). Precision Plus 59 Analysis of.

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A Classroom Laboratory Manual Heather Miller, D. Scott Witherow, Sue Carson and a 10 stock solution are below: Amount Final concentration 1 10 1 10 l Ponceau S stain (Sigma-Aldrich catalog number P), approximately

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Ponceau S (Sigma-Aldrich, catalog number: P) Stain the membrane with Ponceau S solution and cut the strip containing your antigen protein as small as.